Journal: PLOS One

Article Title: Antibacterial property of lead telluride quantum dot layer fabricated on glass substrate

doi: 10.1371/journal.pone.0334629

Figure Lengend Snippet: FTIR spectrum of PbTe QDs.

Article Snippet: FTIR microscope imaging was performed on an infrared microscope (Jasco, IRT-5200–16, Tokyo, Japan) coupled to an FTIR spectrometer (Jasco, 6800 FV, Tokyo, Japan).

Techniques:

Journal: PLoS Pathogens

Article Title: Rotavirus NSP1 Inhibits NFκB Activation by Inducing Proteasome-Dependent Degradation of β-TrCP: A Novel Mechanism of IFN Antagonism

doi: 10.1371/journal.ppat.1000280

Figure Lengend Snippet: (A) MA104 cells were infected with three pfu/cell of the indicated virus strain. Lysates were prepared at 2, 4, 6, 8, and 10 hpi and the abundance of p65 was determined by immunoblot using anti-p65 antibody. Blots were probed with anti-GAPDH as a loading control. (B) The subcellular localization of p65 in MA104 cells infected with three pfu/cell of the indicated virus strain was determined at six hpi by confocal microscopy (63×, NA 1.40). Cells were stained with anti-p65 antibody, followed by Alexa Fluor 594-conjugated goat anti-rabbit IgG.

Article Snippet: The primary antibodies were mouse anti-IRF3 (Santa Cruz Biotechnology) or rabbit anti-p65 (Rockland).

Techniques: Infection, Virus, Western Blot, Control, Confocal Microscopy, Staining

Journal: PLoS Pathogens

Article Title: Rotavirus NSP1 Inhibits NFκB Activation by Inducing Proteasome-Dependent Degradation of β-TrCP: A Novel Mechanism of IFN Antagonism

doi: 10.1371/journal.ppat.1000280

Figure Lengend Snippet: MA104 cells were infected with A5-16, OSU or NCDV at an moi of three pfu/cell. Six hours post-infection, nuclear and cytoplasmic fractions were separated with the nuclear extract kit following the manufacturer's instructions (Active Motif). (A) p65 activation measured by p65 TransAm ELISA. Error bars are the standard error of the mean. (B) Nuclear fractions were probed with anti-p65 antibody, anti-laminA/C (nuclear, BD Biosciences), and anti- GAPDH (cytoplasmic) antibodies.

Article Snippet: The primary antibodies were mouse anti-IRF3 (Santa Cruz Biotechnology) or rabbit anti-p65 (Rockland).

Techniques: Infection, Activation Assay, Enzyme-linked Immunosorbent Assay

Journal: STAR Protocols

Article Title: Protocol to assess the effects of dysfunctional human vascular smooth muscle cells on other brain cells using in vitro models of Alzheimer’s disease

doi: 10.1016/j.xpro.2022.101149

Figure Lengend Snippet: Morphological changes in the cytoskeleton of dysfunctional VSMCs Scale bar: 20 μm

Article Snippet: Culture medium ready-to-use with FBS and antibiotics (VSMCs) , Cell Applications , Cat#311-500.

Techniques:

Journal: STAR Protocols

Article Title: Protocol to assess the effects of dysfunctional human vascular smooth muscle cells on other brain cells using in vitro models of Alzheimer’s disease

doi: 10.1016/j.xpro.2022.101149

Figure Lengend Snippet: Schematic workflow supernatant assay Immunofluorescence staining shows microglia activation after incubation with supernatant from dysfunctional VSMCs. Activated microglial were characterized by morphologic changes and elevated expression of IL-6 at 100 × magnification, scale bars: 5 μm

Article Snippet: Culture medium ready-to-use with FBS and antibiotics (VSMCs) , Cell Applications , Cat#311-500.

Techniques: Immunofluorescence, Staining, Activation Assay, Incubation, Expressing

Journal: STAR Protocols

Article Title: Protocol to assess the effects of dysfunctional human vascular smooth muscle cells on other brain cells using in vitro models of Alzheimer’s disease

doi: 10.1016/j.xpro.2022.101149

Figure Lengend Snippet:

Article Snippet: Culture medium ready-to-use with FBS and antibiotics (VSMCs) , Cell Applications , Cat#311-500.

Techniques: Recombinant, Wound Healing Assay, Staining, SYBR Green Assay, Software, Real-time Polymerase Chain Reaction, Microscopy, Sterility, Inverted Microscopy, Confocal Microscopy